• Users Online: 401
  • Home
  • Print this page
  • Email this page
Home About us Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Login 
ORIGINAL ARTICLE
Year : 2015  |  Volume : 3  |  Issue : 2  |  Page : 74-78

Phenotypic study of Extended-spectrum beta-lactamase, AmpC and Carbapenemase among E.coli clinical isolates in affiliated hospitals of Babol University of Medical Sciences


Department of Laboratory Medicine, School of Medicine, Paramedical Faculty, Infectious and Tropical Disease Research Center, Babol University of Medical Sciences, Mazandaran, Iran

Correspondence Address:
Farahnaz Sadighian
Department of Laboratory Medicine, School of Medicine, Paramedical Faculty, Infectious and Tropical Disease Research Center, Babol University of Medical Sciences, Mazandaran
Iran
Login to access the Email id

Source of Support: This study was financially supported by the Babol University of Medical Sciences, Conflict of Interest: None


DOI: 10.4103/2347-9019.151306

Rights and Permissions

Context: Escherichia coli as a member of Enterobacteriaceae have an important role in infections. Betalactams as a group of antibiotics are used in treatment, widely. Bacteria resist to these antibiotics by producing beta-lactamases. Aims: This study aimed to investigate the phenotypic methods for detecting Extended-spectrum beta-lactamase (ESBL), AmpC and Carbapenemase (Metallobetalactamase (MBL)) in E.coli. Settings and Design: This is a cross-sectional study for detection of beta-lactamases producing E.coli. Subject and Methods: E.coli were isolated from clinical specimens to perform Antimicrobial Susceptibility Test (AST) by disc diffusion method. These bacteria were evaluated for the production of ESBL, AmpC and Metallo-beta-lactamase (MBL) (Imipenem (IMP)-1) enzymes, by using  clinical and laboratory standards institue (CLSI) method and their inhibition by 3-amino-phenyl boronic acid and 2-mercaptopropionic acid, respectively. Also, mask-ESBL production was identified using the different concentration of 3-amino-phenyl boronic acid compound. Statistical Analysis Used: Data were analyzed by the Statistical Package of Social Sciences (SPSS) 18 software. Results: Out of 259 isolated E.coli, 147 (56.8%) isolates were positive for producing at least one type of beta-lactamase. The frequency of ESBL, AmpC and IMP-1 producers were 137 (52.9%), 20 (7.7%) and 80 (31%) isolates, respectively. In addition, 88 (34%) isolates produced two or three types of enzymes simultaneously. Of the 88 isolates, 68 (77.3%) E.coli produced ESBL + IMP-1. Conclusions: This study revealed the high prevalence of beta-lactamase producing E.coli, and there are two or three different types of enzymes in a bacterium.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed4447    
    Printed159    
    Emailed0    
    PDF Downloaded522    
    Comments [Add]    

Recommend this journal